Journal: Microbiology Spectrum

Article Title: Outer membrane vesicles of carbapenem-resistant Acinetobacter baumannii derive an enhanced inflammatory response

doi: 10.1128/spectrum.02627-25

Figure Lengend Snippet: CRAB induces a stronger immune response in mice. Mice were uninfected with 1 × 10 7 colony-forming units (CFUs) of CRAB or A19606 for 6 h ( n = 3), and lungs were harvested and prepared for RNA-sequencing. DEGs were those with an FDR cut-off of 0.05 and a fold change of ≥±2. ( A ) Volcano plot and ( B ) heatmap showed significantly DEGs of the RNA-seq data. ( C ) Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the DEGs. ( D ) Gene Set Enrichment Analysis (GSEA) of the DEGs. ( E ) Mice were uninfected with 1 × 10 7 CFU of CRAB or A19606 for 24 h ( n = 6), qRT-PCR analysis of the gene expression of lung tissues ( n = 6). ( F ) Pathological histological analysis of lung sections stained with hematoxylin and eosin ( n = 6). Scale bar: 250 μm. The data are expressed as the mean ± SD; *** P < 0.001; **** P < 0.0001.

Article Snippet: For comparison, the reference strain A19606 was obtained from the American Type Culture Collection (ATCC).

Techniques: RNA Sequencing, Quantitative RT-PCR, Gene Expression, Staining

Journal: Microbiology Spectrum

Article Title: Outer membrane vesicles of carbapenem-resistant Acinetobacter baumannii derive an enhanced inflammatory response

doi: 10.1128/spectrum.02627-25

Figure Lengend Snippet: CRAB triggers a stronger immune response in macrophages. ( A ) Immunoblot analysis of protein expression in BMDMs treated with CRAB or A19606 for 2 h at a multiplicity of infection (MOI) of 0.1, 1, and 10. ( B ) Gray scale analysis summary of Western blot (WB) results from three independent experiments. ( C ) qRT-PCR analysis of gene expression in BMDMs infected with CRAB for 4 h at an MOI of 10 ( n = 3). The data are expressed as the mean ± SD; * P < 0.05; ** P < 0.01; *** P < 0.001; and **** P < 0.0001; ns, not significant.

Article Snippet: For comparison, the reference strain A19606 was obtained from the American Type Culture Collection (ATCC).

Techniques: Western Blot, Expressing, Infection, Quantitative RT-PCR, Gene Expression

Journal: Microbiology Spectrum

Article Title: Outer membrane vesicles of carbapenem-resistant Acinetobacter baumannii derive an enhanced inflammatory response

doi: 10.1128/spectrum.02627-25

Figure Lengend Snippet: Comparison of protein expression difference between CRAB and A19606. ( A ) Volcano plot showed significantly differentially expressed proteins of CRAB and A19606 by the proteomic analysis. Differentially expressed proteins were those with an FDR cut-off of 0.05 and a fold change of ≥±1.2. ( B ) Subcellular localization analysis and ( C ) GO pathway enrichment analysis of all differentially expressed proteins. Each group had three biological replicates. ( D ) The heatmap showed top 30 highly expressed proteins in CRAB.

Article Snippet: For comparison, the reference strain A19606 was obtained from the American Type Culture Collection (ATCC).

Techniques: Comparison, Expressing

Journal: Microbiology Spectrum

Article Title: Outer membrane vesicles of carbapenem-resistant Acinetobacter baumannii derive an enhanced inflammatory response

doi: 10.1128/spectrum.02627-25

Figure Lengend Snippet: Characteristics of CRAB and A19606 derived OMVs. TEM images of OMVs derived from A19606 ( A ) and CRAB ( B ). Scale bar: 200 nm. NTA of OMVs-A19606 ( C ) and CRAB ( D ).

Article Snippet: For comparison, the reference strain A19606 was obtained from the American Type Culture Collection (ATCC).

Techniques: Derivative Assay

Journal: Microbiology Spectrum

Article Title: Outer membrane vesicles of carbapenem-resistant Acinetobacter baumannii derive an enhanced inflammatory response

doi: 10.1128/spectrum.02627-25

Figure Lengend Snippet: Proteomic analysis of OMVs released by CRAB and A19606. ( A ) Volcano plot showed significantly differentially expressed proteins of OMVs derived from CRAB or A19606 by the proteomic analysis. Differentially expressed proteins were those with a FDR cut-off of 0.05 and a fold change of ≥±1.2. ( B ) Subcellular localization analysis and ( C ) GO pathway enrichment analysis of all differentially expressed proteins (molecular function). ( D ) The heatmap showed top 30 highly expressed proteins in CRAB-derived OMVs.

Article Snippet: For comparison, the reference strain A19606 was obtained from the American Type Culture Collection (ATCC).

Techniques: Derivative Assay

Journal: Microbiology Spectrum

Article Title: Outer membrane vesicles of carbapenem-resistant Acinetobacter baumannii derive an enhanced inflammatory response

doi: 10.1128/spectrum.02627-25

Figure Lengend Snippet: CRAB-derived OMVs trigger a stronger immune response in macrophages. ( A ) Immunoblot analysis of protein expression in BMDMs treated with OMVs-CRAB or OMVs-A19606 for 2 h at 20 μg/mL. ( B ) Gray scale analysis summary of WB results from three independent experiments. ( C ) qRT-PCR analysis of gene expression in BMDMs infected with OMVs-CRAB or OMVs-A19606 for 4 h at an MOI of 10 ( n = 3). The data are expressed as the mean ± SD; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Article Snippet: For comparison, the reference strain A19606 was obtained from the American Type Culture Collection (ATCC).

Techniques: Derivative Assay, Western Blot, Expressing, Quantitative RT-PCR, Gene Expression, Infection